Integrative Center for Environmental Microbiomes and Human Health
Microbial Genomics and Analytical Laboratory
Turnaround time for our services are dependent on the type of service(s) being provided and the number of samples submitted. Our typical processing time for single region amplicon library preparation from DNA extracts for 2-4 plates is approximately 2-3 weeks. DNA extractions are usually completed within 1-2 weeks. If your project deviates from MGAL standard protocols (see the dropdown section below for details), the turnaround time will be extended.
Users can opt to expedite their sample turnaround times.
An expedite fee will be charged in addition to the standard service rates. Please contact us for more information.
To request a formal quote, email us at mgal@hawaii.edu.
Service | University of Hawaii Users | Non-UH Academic & Non-Profit Users | Private & Commercial Users |
---|---|---|---|
DNA Extractions (High throughput – MagAttract PowerSoil Kit) | $19.10/sample (1‐24 samples) $13.50/sample (25‐48 samples) $10.70/sample (49‐96 samples) | $23.85/sample (1‐24 samples) $16.85/sample (25‐48 samples) $13.35/sample (49‐96 samples) | $28.65/sample (1‐24 samples) $20.25/sample (25‐48 samples) $16.05/sample (49‐96 samples) |
DNA Extractions (High throughput – NucleoMag Kit ) | $11.35/sample (1‐24 samples) $7.20/sample (25‐48 samples) $5.15/sample (49‐96 samples) | $14.15/sample (1‐24 samples) $9.00/sample (25‐48 samples) $6.40/sample (49‐96 samples) | $17.00/sample (1‐24 samples) $10.80/sample (25‐48 samples) $7.70/sample (49‐96 samples) |
DNA Extractions (Spin columns) | Variable depending on kit | Variable depending on kit | Variable depending on kit |
Sample Transfer Fee | $2.50/sample | $3.10/sample | $3.75/sample |
qPCR (Probe) | $3.65/sample (1‐24 samples) $2.50/sample (25‐48 samples) $1.90/sample (49‐96 samples) Multiply price by number of replicates | $4.55/sample (1‐24 samples) $3.10/sample (25‐48 samples) $2.35/sample (49‐96 samples) Multiply price by number of replicates | $5.45/sample (1‐24 samples) $3.75/sample (25‐48 samples) $2.85/sample (49‐96 samples) Multiply price by number of replicates |
qPCR (SYBR) | $3.25/sample (1‐24 samples) $2.10/sample (25‐48 samples) $1.50/sample (49‐96 samples) Multiply price by number of replicates | $4.05/sample (1‐24 samples) $2.60/sample (25‐48 samples) $1.85/sample (49‐96 samples) Multiply price by number of replicates | $4.85/sample (1‐24 samples) $3.15/sample (25‐48 samples) $2.25/sample (49‐96 samples) Multiply price by number of replicates |
qPCR Library Quantification | $43.80 for the 1st library $10.00 for each additional library | $54.75 for the 1st library $12.50 for each additional library | $65.70 for the 1st library $15.00 for each additional library |
PCR (16S/18S/ITS) | $4.15/sample (1‐24 samples) $2.55/sample (25‐48 samples) $1.75/sample (49‐96 samples) | $5.15/sample (1‐24 samples) $3.15/sample (25‐48 samples) $2.15/sample (49‐96 samples) | $6.20/sample (1‐24 samples) $3.80/sample (25‐48 samples) $2.60/sample (49‐96 samples) |
Purification and Normalization of PCR Products | $125.00/plate (1‐96 samples) | $156.25/plate (1‐96 samples) | $187.50/plate (1‐96 samples) |
Amplicon Purification (SPRI Bead Cleanup) | $3.50/sample | $4.35/sample | $5.25/sample |
Gel Extraction | $5.50/sample | $6.85/sample | $8.25/sample |
Library Purification (SPRI Bead Cleanup) | $24.00/library | $30.00/library | $36.00/library |
Bioanalyzer (dsDNA HS) | $90.00/chip (up to 11 samples) | $112.50/chip (up to 11 samples) | $135.00/chip (up to 11 samples) |
Agarose gel electrophoresis (with annotation) | $50.00/gel (1‐192 samples) | $62.50/gel (1‐192 samples) | $75.00/gel (1‐192 samples) |
NanoDrop | $1.20/sample | $1.50/sample | $1.80/sample |
Qubit | $2.50/sample | $3.10/sample | $3.75/sample |
Technician Fee | $42.00/hour | $52.50/hour | $63.00/hour |
Instrument Training* | $5.00/instrument | $6.25/instrument | $7.50/instrument |
N2 Evaporator | $10.00/hour | $12.50/hour | $15.00/hour |
Sample Prep | Determined after consultation | ||
GCMS | $15.00/sample | $18.75/sample | $22.50/sample |
DART MS | $20.00/sample | $25.00/sample | $30.00/sample |
New User Setup | $125.00 | $156.25 | $187.50 |
* Excludes Lyophilizer and KingFisher Flex use (requires technician set-up fees). | |||
Rates subject to change (rates effective 07/22/2022) |
The Amplicon Library Preparation Workflow outlines the standard MGAL processes involved in amplicon library preparation
(see the "MGAL Standard Protocols" drop down above for more details). Library preparation protocols vary between labs. You may run into compatibility issues if you sequence an MGAL-prepared amplicon library with samples prepared using different protocols.
For each plate of samples, this amounts to approximately $1,500.00 for 16S, 18S, or ITS libraries.
For pre-extracted samples in plate format, it will be approximately $460.00 for 16S, 18S, or ITS libraries.
1800 East-West Road
Honolulu, HI 96822
microbre@hawaii.edu
808-956-5222