MGAL

Microbial Genomics and Analytical Laboratory

Build a dedicated, centralized facility to provide laboratory infrastructure and services to support COBRE ICEMHH project
– To support the research aims of individual COBRE ICEMHH labs
– To expand and promote research on the microbial diversity found in native Hawaiian fauna and ecosystems
– High-throughput genomic library preparation from environmental samples
– Natural product and small molecule analysis
– Culturing and storage of microbial strains
– Integration with C-MĀIKI bioinformatic pipeline, data sharing, and storage
Microbial Genomics
– Nucleic acid extraction
– Amplicon generation (16S/ITS/Custom)
– Library preparation
– Pre-sequencing quality control

Small Molecule Analysis
– DART MS small molecule analysis
– GCMS analysis
– Thin layer chromatography
– Fatty acid quantitation
– Amino acid quantitation
– Lipid extraction from biological samples
Analytical Lab
– Ambient ionization mass spectrometer
– Gas chromatography mass spectrometer
– Nitrogen evaporator

Genomics
– Bioanalyzer
– Gel imager
– Kingfisher Flex (automated nucleic acid purification unit)
– Lyophilizer
– Multiskan Sky Microplate Spectrophotometer
– Sample homogenizer
– Thermal cycler
– qPCR machine
– Qubit

General Use
– Autoclave
– Biosafety cabinet
– Centrifuge
– Cold storage (4°C, -20°C, -80°C)
– Incubator
– Shaker

Location

University of Hawaii at Mānoa
1800 East-West Road
LSB Room 203
Honolulu, HI 96822

Contact

Phone: 808-956-2713

Email: ny9@hawaii.edu or kkc25@hawaii.edu